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实验用北京鸭12只,4～5周龄,体重约1.5kg左右,雌雄不限.用慢性手术方法,往鸭第Ⅲ脑室内微量注0.1％秋水仙素,24小时后,将动物灌流,取下丘脑,切片后用免疫组织化学方法观察鸭下丘脑CCK神经元的分布.结果表明,下丘脑室旁核、正中隆起及腹外侧区均存在CCK免疫标记细胞。室旁核的CCK神经元多见于大细胞神经元,而正中隆起和腹外侧区CCK神经元胶体多在血管周围,下丘脑腹内侧核未见CCK阳性标记细胞. 另选同上条件北京鸭18只,在肌胃表面慢性埋植Ag-Agcl乏极化电极一对.待动物恢复后,将鸭头前倾45°,固定在立体定位仪上,按前囟前4mm、旁开0.5mm处.埋内径为0.45mm,长7mm不锈钢导向管,再将尖端为30μm左右微玻管通过导向管下移至VMH(距脑表面13.5mm),微玻管另一端通过硅胶管与10μI微量注射
The experimental Beijing duck 12, 4 to 5 weeks of age, weighing about 1.5kg, male or female.Use chronic surgery method, to duck third micro ventricle 0.1% colchicine, 24 hours later, the animals were perfused, The thalamus was removed and the distribution of CCK neurons in the hypothalamus was observed by immunohistochemistry after slicing.The results showed that there were CCK immunofluorescent markers in the paraventricular nucleus of the hypothalamus, midlift and ventral region. Paraventricular nucleus CCK neurons more common in large cell neurons, while the median bulge and ventral CCK neuronal colloid in the blood vessels around the hypothalamic ventromedial nucleus CCK positive marker cells.Optionally the same conditions Beijing duck 18 , A pair of Ag-Agcl depolarized electrodes were implanted chronicly on the surface of muscular stomach, and after the animals were recovered, the ducks were tilted 45 ° anteriorly and fixed on a stereotaxic instrument, 4 mm anterior to the bregma and 0.5 mm apart. Buried 0.45mm in diameter, 7mm long stainless steel guide tube, and then the tip of about 30μm microgrooves through the guide tube down to the VMH (13.5mm from the brain surface), the other end of the microglass tube through a silicone tube with 10μI microinjection