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在常规的Zarrouk培养基中添加1000∶3(v/v)的罗非鱼下脚料蛋白酶解液对钝顶螺旋藻进行混合营养培养,考察在光照强度230μmol/m2.s、温度15~40℃范围内对螺旋藻生物量、叶绿素、藻胆蛋白和蛋白质含量的影响。结果表明,添加酶解液在15~35℃时能够显著提高螺旋藻生物量,35℃时增幅最大为38.2%;在30~40℃时添加酶解液显著提高螺旋藻叶绿素的含量,30℃时含量提高了11.7%;在15~20℃低温条件下添加酶解液显著提高螺旋藻藻胆蛋白含量,15℃时增幅达15.1%;在15~35℃范围内添加酶解液显著提高蛋白质含量,30℃时增幅最大为13.7%。
In a conventional Zarrouk medium supplemented with 1000: 3 (v / v) tilapia leftover protein hydrolyzate mixed culture of Spirulina platensis were investigated at a light intensity of 230μmol / m2.s, temperature 15 ~ 40 ℃ On the biomass of Spirulina, chlorophyll, phycobiliprotein and protein content in the range. The results showed that the biomass of spirulina could be significantly increased by adding enzyme solution at 15-35 ℃, and the maximum increment was 38.2% at 35 ℃. Adding enzyme solution at 30-40 ℃ significantly increased the content of chlorophyll at 30 ℃ The content of phycobiliproteins was increased by 11.7% at 15 ~ 20 ℃. The content of phycobiliproteins was increased by 15.1% at 15 ℃. The addition of hydrolyzate at 15 ~ 35 ℃ significantly increased the protein Content, the largest increase at 30 ℃ was 13.7%.