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目的:探讨白藜芦醇(RES)在脊髓损伤(SCI)早期对细胞色素C氧化酶(COX)和超氧化物歧化酶(SOD)活性的影响。方法:2008年2月~2008年8将54只SD健康成年雄性大鼠随机分成3组,根据Allen’s法制成中度脊髓损伤模型,术后立即管饲白藜芦醇100mg/kg或甲基强的松龙(MPSS)100mg/kg;通过比色法观察脊髓损伤8、24及72h后白藜芦醇组脊髓COX及SOD活性的变化,并与MPSS(甲基强的松龙)组进行疗效对比。结果:在脊髓损伤后8、24及72h白藜芦醇组COX活性与损伤组比较差异均有统计学意义,显示白藜芦醇对脊髓损伤具有明显干预作用,而且白藜芦醇组与MPSS组间仅损伤后72h差异无统计学意义(P>0.05);SOD活性在脊髓损伤后8、24及72h白藜芦醇组及MPSS组与损伤组比较差异均有统计学意义,表明白藜芦醇可以提高SOD活性,但在脊髓损伤后8及72h白藜芦醇组与MPSS组比较差异无统计学意义(P>0.05),损伤后24h两者间比较差异有统计学意义。结论:白藜芦醇在脊髓损伤早期能够有效上调COX和SOD活性,对损伤后脊髓起保护作用。
Objective: To investigate the effect of resveratrol on the activity of cytochrome C oxidase (COX) and superoxide dismutase (SOD) in the early stage of spinal cord injury (SCI). METHODS: From February 2008 to August 2008, 54 SD adult male rats were randomly divided into three groups. According to Allen’s method, a moderate spinal cord injury model was established. Resveratrol (100 mg / kg) or methyl group (MPSS) 100mg / kg. The changes of COX and SOD activity in the spinal cord of resveratrol group were observed by colorimetry at 8, 24 and 72 hours after spinal cord injury, and the results were compared with MPSS (methylprednisolone) group Compared. Results: There were significant differences in the activity of COX between resveratrol group and injury group at 8, 24 and 72 h after spinal cord injury, which showed that resveratrol had a significant intervention on spinal cord injury, and resveratrol group and MPSS There was no significant difference between the two groups at 72h after injury (P> 0.05). The SOD activity was significantly different between resveratrol group and MPSS group at 8, 24 and 72 h after SCI However, there was no significant difference between resveratrol group and MPSS group at 8 and 72 h after spinal cord injury (P> 0.05), and there was significant difference between the two groups at 24h after injury. CONCLUSION: Resveratrol can effectively up-regulate the activity of COX and SOD in the early stage of spinal cord injury and protect the spinal cord after injury.