应用间接免疫荧光法和显微荧光分光光度技术，研究了试管内无细胞毒性浓度的亚硒酸纳（硒）对来源于伯基特淋巴瘤的Ｒａｊｉ细胞株，ＥｐｓｔｅｉｎＢａｒ病毒（ＥＢＶ）抗原表达的影响以及抑制ＥＢＶ转化人脐血Ｂ淋巴细胞（ＢＬ）的作用。结果表明，００１～０１ｍｇ／Ｌ的硒使Ｒａｊｉ细胞的ＥＡ抗原抑制３５３％～５０１％；００１～０５ｍｇ／Ｌ的硒令ＥＢＮＡＭＦＳ值降低８４％～２１８％；０１～１０％ｍｇ／Ｌ的硒明显抑制ＥＢＶ转化ＢＬ，抑制率为６３３％～９２１％。由于ＥＢＶ与鼻咽癌（ＮＰＣ）密切相关，ＮＰＣ病人血清和头发中硒含量明显低于健康人。本研究提示：对ＮＰＣ患者和ＥＢＶ基因表达活跃的人群适当补硒，可能有助于ＮＰＣ和与ＥＢＶ相关疾病的预防和辅助治疗。 Indirect immunofluorescence and microscopic fluorescence spectrophotometry were used to study the in vitro cytotoxic concentration of selenite (selenium) against Raji cell line derived from Burkitt’s lymphoma and EpsteinBar virus (EBV) antigen. The effects of expression and inhibition of EBV transformed human umbilical cord blood B lymphocytes (BL). The results showed that 001～01mg/L selenium inhibited the EA antigen in Raji cells by 353%～501%; 001～05mg/L selenium reduced the EBNAMFS value by 8. 4%～218%; 01～10%mg/L selenium significantly inhibited the conversion of EBV to BL, and the inhibition rate was 633%-921%. Because EBV is closely related to nasopharyngeal carcinoma (NPC), the selenium content in serum and hair of NPC patients is significantly lower than that of healthy individuals. This study suggests that appropriate supplementation of selenium in NPC patients and those with active EBV gene expression may contribute to the prevention and adjuvant treatment of NPC and EBV-related diseases.