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应用21对SSR引物与毛细管电泳技术,分析了52个甘蔗属品种的遗传多样性。共检测出327个SSR标记,平均每对引物检测15.6个。选择141个共显性标记构建SSR标记指纹图谱数据库,利用DNAMAN软件与UPGMA统计方法分析参试材料遗传多样性。DNAMAN软件同源分析显示,新台糖16号与台优1号之间的同源性最高(87%),品种之间最小的同源性为55%;利用UPGMA统计方法可把参试材料分成4个遗传相似性较高的类群。结果表明,SSR标记与毛细管技术的结合,可构建甘蔗种质资源SSR标记指纹图谱、分析甘蔗种质资源遗传多样性。聚类分析显示参试甘蔗材料的遗传基础相近,为了提高甘蔗选育种效率,应拓宽甘蔗选育种亲本的遗传基础,提高杂交栽培品种的抗虫、抗病等特性。
21 pairs of SSR primers and capillary electrophoresis were used to analyze the genetic diversity of 52 sugarcane varieties. A total of 327 SSR markers were detected, with an average of 15.6 for each pair of primers. A total of 141 co-dominant markers were selected to construct the SSR fingerprinting database. The DNAMAN software and UPGMA statistical methods were used to analyze the genetic diversity of the tested materials. DNAMAN software homology analysis showed that the highest homology between Xintai 16 and Taiyou 1 was 87%, and the minimum homology between varieties was 55%. According to UPGMA statistical method, Four groups with higher genetic similarity. The results showed that the combination of SSR markers and capillary technology could construct the SSR fingerprinting of sugarcane germplasm resources and analyze the genetic diversity of sugarcane germplasm resources. Cluster analysis showed that the genetic basis of the tested sugarcane materials was similar. In order to improve the efficiency of breeding sugarcane, the genetic basis of the parents of sugarcane breeding should be broadened, and the characteristics of insect-resistant and disease-resistance of hybrid cultivars should be improved.