用高效液相色谱法对大黄虫胶囊中大黄酸进行了含量测定，采用ＹＷＧ－Ｃ１８（５μｍ）４６ｍｍ×２５０ｍｍ色谱柱，以甲醇—水—冰醋酸（３３０∶１７０∶５）为流动相，检测波长２５４ｎｍ，柱温４０℃，本方法回收率为９７９５％，ＲＳＤ为２８３％，是一种简便、快速、准确的测定方法。 The contents of rhein in Rhubarb capsule were determined by high performance liquid chromatography (HPLC), using YWG-C18 (5μm) 46mm × 250mm column with methanol-water-glacial acetic acid (330:170:5) The detection wavelength was 254 nm and the column temperature was 40 ℃. The recovery rate of this method was 9795% with RSD of 283%, which was a simple, rapid and accurate method for determination.