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目的 :研究外源性骨髓间充质干细胞(BMSCs)对大肠埃希菌致大鼠前列腺炎的抑制作用。方法:贴壁选择法分离、培养、扩增BMSCs。将SD大鼠50只随机分为急性细菌性前列腺炎(ABP)组、ABP+BMSCs组、慢性细菌性前列腺炎(CBP)组、CBP+BMSCs组和正常对照组,每组10只。在小动物超声引导下向大鼠前列腺两侧叶内注射大肠埃希菌建立前列腺炎症模型,注入细菌1~14 d为急性炎症期,4~12周为慢性炎症期,对照组注入等量PBS。然后将BMSCs注入ABP+BMSCs组和CBP+BMSCs组大鼠前列腺内,观察移植BMSCs2周后大鼠前列腺病理学变化并做炎症评分,RT-PCR扩增前列腺组织中炎症因子IL-1β、TNF-αmRNA,ELISA检测IL-1β、TNF-α蛋白含量,统计分析各组表达差异。结果:前列腺组织病理学提示炎症组前列腺组织呈典型炎症病理变化,腺管结构改变,间质水肿,炎细胞浸润,纤维组织增生,而BMSCs治疗组大鼠前列腺炎症明显减轻。PCR和ELISA分析显示ABP组IL-1βmRNA(0.829±0.121)、蛋白(271.75±90.59)pg/ml和TNF-αmRNA(0.913±0.094)、蛋白(105.78±19.05)pg/ml含量均显著高于对照组[(0.342±0.087)、(45.76±17.99)pg/ml]、[(0.247±0.054)、(19.42±7.75)pg/ml](P均<0.01)及BMSCs治疗组[(0.433±0.072)、(51.34±22.13)pg/ml]、[(0.313±0.076)、(28.38±8.78)pg/ml](P<0.01);CBP组IL-1βmRNA(0.975±0.114)、蛋白(265.31±71.34)pg/ml和TNF-αmRNA(0.886±0.084)、蛋白(107.45±26.11)pg/ml含量也均显著高于对照组及BMSCs治疗组[(0.396±0.064)、(56.37±21.22)pg/ml]、[(0.417±0.068)、(29.21±10.22)pg/ml](P均<0.01)。结论:注入BMSCs能够减轻大肠埃希菌引起的前列腺炎症反应,其作用可能与减少炎细胞浸润,降低IL-1β、TNF-α水平有关。
Objective: To study the inhibitory effect of exogenous bone marrow mesenchymal stem cells (BMSCs) on prostatitis induced by Escherichia coli in rats. Methods: Adherent selection method was used to isolate, culture and amplify BMSCs. 50 SD rats were randomly divided into acute bacterial prostatitis (ABP) group, ABP + BMSCs group, chronic bacterial prostatitis (CBP) group, CBP + BMSCs group and normal control group, 10 rats in each group. The model of prostatic inflammation was established by injecting Escherichia coli into the leaves of both sides of the prostate of rats under the guidance of ultrasound of small animals. The bacteria were injected for 1 ~ 14 days for acute inflammation and 4 ~ 12 weeks for chronic inflammation. The control group was injected PBS . BMSCs were then injected into the prostate of ABP + BMSCs group and CBP + BMSCs group. The pathological changes of prostate in 2 weeks after BMSCs transplantation were observed and scored. Inflammatory cytokines were evaluated by RT-PCR. The expressions of IL-1β, TNF- αmRNA, ELISA detection of IL-1β, TNF-α protein content, statistical analysis of the differences in expression of each group. Results: Prostate histopathology suggested that inflammation of the prostate tissue showed typical inflammatory pathological changes, structural changes of the duct, interstitial edema, infiltration of inflammatory cells and fibrous tissue hyperplasia. However, prostatitis in the BMSCs-treated group was significantly reduced. PCR and ELISA analysis showed that the levels of IL-1β mRNA (0.829 ± 0.121), protein (271.75 ± 90.59) pg / ml and TNF-αmRNA (0.913 ± 0.094) and protein (105.78 ± 19.05) pg / ml in ABP group were significantly higher than those in control (0.342 ± 0.087), (45.76 ± 17.99) pg / ml], [(0.247 ± 0.054), (19.42 ± 7.75) pg / ml] , (51.34 ± 22.13) pg / ml], [(0.313 ± 0.076), (28.38 ± 8.78) pg / ml] pg / ml and TNF-αmRNA (0.886 ± 0.084) and protein (107.45 ± 26.11) pg / ml were significantly higher than those in the control group and the BMSCs treated group [(0.396 ± 0.064), (56.37 ± 21.22) pg / ml] , [(0.417 ± 0.068), (29.21 ± 10.22) pg / ml] (all P <0.01). CONCLUSION: Injection of BMSCs can reduce the inflammatory response induced by Escherichia coli, which may be related to the reduction of inflammatory cell infiltration and the decrease of IL-1β and TNF-α levels.