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有关人类精子冷冻贮存方法的研究已有不少报道,但现有方法大都需要专用降温冷冻设备或辅助装置,因此在基层医疗单位应用不无困难。我们从1986年5月开始,对精子冷冻贮存方法进行了研究,经过近两年的实践,初步总结了一个简易的人类精子冷冻贮存方法,现介绍如下。一、设备与药品液氮罐(10公升或30公升),玻璃试管(10×75mm),自制小纱布袋(长7 cm,宽3～4 cm,袋口系吊线),注射器(5 ml 或10ml),玻璃棒(长40cm,标有 cm 刻度),细玻璃棒(长12～15cm,或眼科滴眼玻棒),烧杯或量杯(100ml),广口小瓶,镊子,止血钳,药物天平,甘油(分析纯),枸椽酸钠,红霉素,液氮,新鲜鸡蛋等。二、致冷源
There have been many reports on the methods of human sperm cryopreservation. However, most existing methods require special cooling and freezing equipment or ancillary devices. Therefore, it is not difficult to apply them in primary medical units. We started from May 1986 on sperm cryopreservation methods were studied, after nearly two years of practice, initially summed up a simple cryopreservation of human sperm, are presented below. Equipment and Drugs Liquid nitrogen tank (10 liters or 30 liters), glass test tubes (10 × 75 mm), self-made gauze bags (7 cm long, 3 cm to 4 cm wide, 10ml), glass rod (length 40cm, marked with a scale), thin glass rod (length 12 ~ 15cm, or eye drops), beaker or measuring cup (100ml), wide mouth vials, tweezers, , Glycerin (analytical grade), sodium citrate, erythromycin, liquid nitrogen, fresh eggs and so on. Second, the cooling source