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发酵法生产泰乐菌素过程中产生的药渣,因残留泰乐菌素的存在,会对环境造成不利影响。采用富集驯化方法,从堆放泰乐菌素药渣附近土壤中筛选到1株高效降解泰乐菌素的菌株,经16S rDNA鉴定为无丙二酸柠檬酸杆菌(Citrobacter amalonaticus)。该菌产生的降解泰乐菌素的主要酶是胞内组成酶。单因素实验结果显示,该酶降解泰乐菌素适宜的条件为温度35℃、pH 5.5、加酶量12%和初始底物浓度低于20 mg/L。多因素正交实验结果显示,温度对泰乐菌素的降解率影响较大。
The dregs produced in the fermentative process for the production of tylosin can have an adverse effect on the environment due to the presence of residual tylosin. Using enrichment and acclimation method, a strain of high tylosin-degrading strain was screened from the soil near tylosin dregs, and identified by 16S rDNA as Citrobacter amalonaticus. The main enzyme for the degradation of tylosin produced by this bacterium is intracellular enzyme. The results of single factor experiments showed that the optimal conditions for the degradation of tylosin by this enzyme were temperature 35 ℃, pH 5.5, enzyme addition 12% and initial substrate concentration 20 mg / L. The results of multivariate orthogonal experiment showed that the temperature had a great effect on the degradation rate of tylosin.