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目的:检测辣椒碱、姜黄素、银杏叶提取物、茶多酚、青石莲提取物对4HNE(4羟基壬烯醛)诱导的人表皮黑素细胞氧化损伤的保护作用,比较5种植物提取物保护4羟基壬烯醛损伤黑素细胞作用的强弱。方法:4HNE损伤黑素细胞,WTS法测细胞活性,流式细胞仪测细胞内活性氧(ROS)水平及细胞凋亡率,ABTS测细胞总的抗氧化能力。结果:50μmol/L 4HNE处理后,细胞活性大约下降至对照组50%~60%(P<0.05),筛选最佳作用浓度辣椒碱5μmol/L、姜黄素4μmol/L、银杏叶提取物40μmol/L、茶多酚50μg/m L、青石莲提取物40μg/m L,此5种植物提取物对50μm 4HNE诱导的黑素细胞损伤都有保护作用。其中,增强黑素细胞活性最强的是姜黄素(P<0.05)、降低细胞内活性氧最强的是茶多酚(P<0.01)、降低细胞凋亡率最强的是银杏叶(P<0.05)、提高黑素细胞总的抗氧化能力辣椒碱的作用最强(P<0.05)。对4羟基壬烯醛损伤黑素细胞的保护作用由强到弱排序:辣椒碱、银杏叶和茶多酚。结论:此5种植物提取物均可保护4HNE损伤的黑素细胞,提高细胞抗氧化活性,增强黑素细胞活性,这可能为其临床治疗白癜风提供理论依据。
OBJECTIVE: To test the protective effect of capsaicin, curcumin, ginkgo biloba extract, tea polyphenol, and Qingshi Lian on the oxidative damage induced by 4HNE (4-hydroxynonenal) in human epidermal melanocytes. The protective effect of 4-hydroxynonenal on the function of melanocytes. Methods: The melanocytes were stained with 4HNE, the cell viability was measured by WTS, the level of intracellular reactive oxygen species (ROS) and cell apoptosis were measured by flow cytometry. The total antioxidant capacity of ABTS cells was measured. Results: After 50μmol / L 4HNE treatment, the cell viability decreased to about 50% -60% in the control group (P <0.05). The optimal concentrations of 5μmol / L capsaicin, 4μmol / L curcumin and 40μmol / L, 50μg / m L of tea polyphenols and 40μg / m L of Qingshuilian extract. These 5 kinds of plant extracts have protective effects on 50μm 4HNE-induced melanocyte injury. Among them, the strongest activity of melanocytes was curcumin (P <0.05), the strongest activity of reducing intracellular reactive oxygen species was polyphenols (P <0.01), and the strongest apoptosis rate was melanocyte (P <0.05), and the effect of enhancing the total antioxidant capacity of melanocytes was the strongest (P <0.05). The protective effects of 4-hydroxynonenal on melanocytes were ranked from strong to weak: capsaicin, ginkgo biloba and tea polyphenols. CONCLUSION: All five plant extracts can protect melanocytes damaged by 4HNE, enhance cell anti-oxidative activity and enhance melanocyte activity, which may provide a theoretical basis for its clinical treatment of vitiligo.