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目的 研究脑梗死病灶周围及海马处神经前体细胞增殖水平的动态变化。方法 采用易卒中型肾性高血压大鼠 (RHRSP) ,电凝大脑中动脉 (MCA )主干制成脑梗死 (MCAO)模型。行大鼠神经功能评定 ,免疫组化观察并计数梗死灶边缘、对侧镜区及双侧海马 5 -溴脱氧尿核苷 (Bromodeoxyuridine,Brd U )标记的细胞。结果 MCAO后大鼠神经功能评分减低 ,5 d时恢复正常。 MCAO后梗死灶边缘、对侧镜区及双侧海马均有 Brd U阳性细胞分布 ,且病灶侧多于病灶对侧 ,集中分布于病灶周围。结论 脑缺血可诱导神经前体细胞增殖并移向病灶 ,可能成为脑梗死恢复的重要物质基础
Objective To study the dynamic changes of the proliferation of neural precursor cells around the cerebral infarction and in the hippocampus. Methods Stroke-prone renal hypertensive rats (RHRSP) and electrocoagulation of the middle cerebral artery (MCA) were used to establish a cerebral infarction (MCAO) model. The neurological function was evaluated by immunohistochemistry. Bromodeoxyuridine (BrdU) labeled cells were observed at the margins of the infarct, contralateral mirror area and bilateral hippocampus. Results The score of neurological function decreased after MCAO and returned to normal after 5 days. After MCAO, the BrdU positive cells were distributed on the edge of the infarct, the contralateral mirror area and the bilateral hippocampus, and the lesion side was more than the contralateral side of the lesion, which was concentrated around the lesion. Conclusion Cerebral ischemia can induce the proliferation of neural precursor cells and move to the lesion, which may be an important material basis for the recovery of cerebral infarction