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目的 探讨凋亡相关基因 bcl- 2过表达对乙醇诱导的肝细胞癌 (HCC)细胞凋亡的影响 .方法 将含有人 bcl- 2 c DNA的逆转录病毒表达载体 p DOR- SB质粒转染到不表达 bcl- 2蛋白的人肝癌细胞系 HCC- 92 0 4细胞中 ,用免疫组织化学 ABC法检测bcl- 2蛋白的表达情况 .连续克隆化直至
Objective To investigate the effect of apoptosis-related gene bcl-2 overexpression on the apoptosis of hepatocellular carcinoma (HCC) cells induced by ethanol. METHODS: The retroviral expression vector p DOR-SB plasmid containing human bcl-2 c DNA was transfected into In the human hepatocellular carcinoma cell line HCC-92 04 cells that do not express bcl-2 protein, the expression of bcl-2 protein was detected by immunohistochemistry ABC method. Continuous cloning was performed until