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目的:观察2-甲氧基雌二醇(2ME2)对肝癌huh7细胞增殖及凋亡的影响,并初步探讨其分子机制。方法:采用不同浓度的2ME2作用于肝癌huh7细胞,以噻唑蓝(MTT)法测定其细胞毒作用;流式细胞仪检测细胞周期和细胞凋亡;Western blot观察血管内皮生长因子(VEGF)及Bcl-2蛋白的表达。结果:2ME2可抑制肝癌细胞huh7的生长,具有浓度和时间依赖性;2ME2能抑制huh7细胞分裂,大部分细胞阻滞在G2/M期,S期细胞减少;2ME2能诱导huh7细胞凋亡,主要是诱导huh7细胞发生早期凋亡;免疫印迹检查结果显示,2ME2能下调VEGF及Bcl-2蛋白的表达。结论:2ME2能抑制肝癌细胞huh7的增殖并诱导其凋亡,其机制可能与通过下调VEGF及Bcl-2表达有关。
Objective: To observe the effect of 2-methoxyestradiol (2ME2) on the proliferation and apoptosis of human hepatocellular carcinoma huh7 cells and to explore its molecular mechanism. Methods: The human hepatocellular carcinoma huh7 cells were treated with different concentrations of 2ME2, and the cytotoxicity was determined by MTT assay. The cell cycle and apoptosis were detected by flow cytometry. The expressions of VEGF and Bcl - -2 protein expression. Results: 2ME2 inhibited the growth of huh7 cells in a concentration-and time-dependent manner. 2ME2 inhibited huh7 cell division, most of the cells arrested in G2 / M phase and decreased in S phase. 2ME2 induced huh7 cell apoptosis, mainly Induced early apoptosis in huh7 cells. Immunoblotting showed that 2ME2 downregulated the expression of VEGF and Bcl-2. Conclusion: 2ME2 can inhibit the proliferation and induce the apoptosis of huh7 cells, which may be related to the down-regulation of VEGF and Bcl-2 expression.