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在硼砂-HCl(pH=8.2)的水溶液中,α-萘酚(α-N)和β-萘酚(β-N)分别与β-环糊精(β-CD)形成包合物,对共振瑞利散射光谱(RRS)有很强的增敏作用。实验发现,α-N和β-N两种包合物的RRS光谱在320nm处有等强度的等散射点,β-N的包合物在338nm处出现一个突出尖峰,经偏振实验证实是共振荧光峰,而两者在470nm处的共振瑞利散射峰形和增强趋势一致,且具加和性。故可在470nm处测定萘酚的总量,再在338nm处以“同原射线计量法”测定β-N和α-N的含量。在470nm处测定萘酚总量实验的线性范围为5.0×10-6~3.6×10-4mol/L,最低检出限为2.0×10-6mol/L,RSD为3.5%。在338nm处测定β-N和α-N含量的线性范围均为5.0×10-6~3.1×10-4mol/L,最低检出限分别为1.6×10-6mol/L和1.8×10-6mol/L,RSD分别为3.7%和3.6%。测定的选择性较好,据此可对合成样品进行同时测定和对α-萘酚试剂的纯度分析,结果令人满意。
In aqueous solution of borax-HCl (pH = 8.2), α-naphthol (β-N) and β-naphthol (β-N) Resonance Rayleigh scattering spectroscopy (RRS) has a strong sensitizing effect. It was found that the RRS spectra of α-N and β-N inclusion complexes have isosceles scattering points at 320 nm, and the inclusion complex of β-N showed a prominent spike at 338 nm, which was confirmed by polarization experiment Fluorescence peak, and the two at 470nm resonance Rayleigh scattering peak shape and enhance the same trend, and with additivity. Therefore, the total amount of naphthol can be measured at 470 nm, and then measured at 338 nm with the “original radiation metrology” determination of β-N and α-N content. The linear range of the determination of total naphthol at 470nm was 5.0 × 10-6 ~ 3.6 × 10-4mol / L, the minimum detection limit was 2.0 × 10-6mol / L, RSD was 3.5%. The linear range of determination of β-N and α-N at 338 nm ranged from 5.0 × 10-6 to 3.1 × 10-4 mol / L with the detection limits of 1.6 × 10-6 mol / L and 1.8 × 10-6 mol / L, RSD 3.7% and 3.6% respectively. The selectivity of the determination is better, and therefore the simultaneous determination of the synthetic samples and the purity analysis of the α-naphthol reagent are satisfactory.