L-精氨酸对脂多糖诱导免疫应激大鼠血清生化指标和肝脏的影响

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目的探讨外源性精氨酸对动物机体在免疫应激状态下的保护作用。方法以腹腔注射大肠杆菌内毒素(LPS)的方式复制免疫应激动物模型。将SD大鼠32只随机分成4组:正常对照组;生理盐水配对组;免疫应激组(LPS组);L-精氨酸+LPS组(L-Arg+LPS组)。结果腹腔注射LPS 4mg/kg.bw,可以使大鼠产生免疫应激。L-Arg+LPS组大鼠血清中Arg浓度非常显著高于其余各组(P<0.01),与对照组相比,L-Arg+LPS组和LPS组血液中谷丙转氨酶(ALT)活性非常显著(P<0.01),L-Arg+LPS组和LPS组血清中总的一氧化氮合酶(T-NOS)活性显著增高(P<0.05),NO浓度和诱导型一氧化氮合酶(iNOS)活性达到非常显著(P<0.01)。免疫组化显示:L-Arg+LPS组的大鼠肝组织内皮型一氧化氮合酶(eNOS)表达呈强阳性,其他组未见eNOS表达;而LPS组肝组织iNOS表达呈中度阳性,L-Arg+LPS组iNOS表达呈痕迹反应,对照组和配对组均无iNOS表达。L-Arg+LPS组和LPS组分别与对照组相比,肝细胞凋亡极显著增多(P<0.01),但L-Arg+LPS组细胞凋亡数显著低于LPS组(P<0.05),对照组和配对组之间差异不显著。结论对免疫应激大鼠添加精氨酸后,可抑制由iNOS催化生成NO而导致的肝脏组织炎症及肝细胞凋亡,缓减免疫应激对大鼠机体的损害作用。 Objective To investigate the protective effect of exogenous arginine on the immune system of animals. Methods The animal model of immune stress was reproduced by intraperitoneal injection of E. coli lipopolysaccharide (LPS). 32 SD rats were randomly divided into 4 groups: normal control group, saline paired group, immune stress group (LPS group) and L-arginine + LPS group (L-Arg + LPS group). The results of intraperitoneal injection of LPS 4mg / kg.bw, rats can produce immune stress. Arg + LPS group serum Arg concentration was significantly higher than the other groups (P <0.01), compared with the control group, L-Arg + LPS group and LPS group alanine aminotransferase (ALT) activity was very significant (P <0.01). The levels of total nitric oxide synthase (T-NOS) in L-Arg + LPS group and LPS group were significantly increased (P <0.05) ) Activity reached very significant (P <0.01). Immunohistochemistry showed that the expression of eNOS in liver tissue of L-Arg + LPS group was strongly positive, while eNOS expression was not found in the other groups. The expression of iNOS in LPS group was moderately positive, The expression of iNOS in L-Arg + LPS group showed a trace reaction, but iNOS expression was not found in the control group and the matched group. L-Arg + LPS group and LPS group compared with the control group, the apoptosis of hepatocytes was significantly increased (P <0.01), but the apoptosis of L-Arg + LPS group was significantly lower than that of LPS group (P <0.05) There was no significant difference between the control group and the matched group. Conclusion Arginine can reduce the inflammation of liver tissue and the apoptosis of liver cells induced by iNOS-induced nitric oxide synthase (NO) -induced immune damage in rats and attenuate the damaging effect of immune stress on the body of rats.
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