论文部分内容阅读
目的通过比较成年巴马小型猪和贵州小型猪的冠状动脉分型、血管弯曲度、左、右冠状动脉直径和植入支架直径,长期饲养后动物体重变化,探讨两种品系小型猪在冠状动脉支架评价中的应用。方法巴马小型猪9只,贵州小型猪12只,雌雄兼用。常规麻醉动物后,称重,行冠状动脉造影,目测冠脉直径,以过膨胀方式植入支架,再次行冠脉造影观察支架贴壁情况。术后常规饲养动物,饲喂阿司匹林和氯吡格雷抗凝。于支架植入后26周,再次麻醉动物,称重,行安乐死。比较两次实验动物体质量变化,由介入医师根据冠状动脉造影图像分析冠脉分型、血管弯曲度,采用图像分析软件测量植入支架段冠状动脉的最小直径和参考直径。结果两组动物均完成支架植入实验并存活至实验终点,死亡率0%。经过26周饲养,巴马小型猪的体质量变化显著小于贵州小型猪(P<0.001)。两种小型猪冠状动脉均为右优势型,血管弯曲度<30°。巴马小型猪的左冠状动脉前降支的参考直径和最小直径以及植入支架型号与贵州小型猪无统计学差异(P>0.05)。巴马小型猪的右冠状动脉的参考直径明显小于贵州小型猪(P<0.05),最小直径和植入支架型号无统计学差异(p>0.05)。结论利用活体、动态的冠状动脉造影分析,发现巴马和贵州小型猪的成年动物冠状动脉分型均一且与人类一致;血管弯曲度小、易于进行介入导管操作;主要分支直径适合植入直径2.75 mm或3.0 mm规格的冠脉支架。在慢性实验饲养过程中,成年动物的体重变化不大。因此,两种品系小型猪均适合冠脉支架评价研究。
Objective To compare the changes of coronary artery type, vascular curvature, diameter of left and right coronary arteries and diameter of implantable stents in adult Bama miniature pigs and Guizhou miniature pigs, and the change of body weight of animals after long-term feeding to investigate the effects of two strains of miniature pigs in coronary artery Application of scaffold evaluation. Methods Bama minipigs 9, Guizhou miniature pigs 12, both male and female. After routine anesthesia, the animals were weighed, coronary angiography was performed, the diameter of coronary arteries was visualized, the stent was over-inflated, and coronary angiography was performed again to observe the adhension of the stent. Animals were routinely housed and were given aspirin and clopidogrel anticoagulation. At 26 weeks after stent implantation, the animals were anesthetized again, weighed and euthanized. The changes of body weight were compared between two experimental animals. Coronary angiography and vessel tortuosity were analyzed by interventional physicians according to coronary angiography images. The minimum diameter and reference diameter of coronary artery implanted with stents were measured by image analysis software. Results Both groups of animals completed the stent implantation experiment and survived to the end of the experiment with a mortality rate of 0%. After 26 weeks of feeding, Bama miniature pigs had significantly lower body weight changes than Guizhou miniature pigs (P <0.001). Both miniature pig coronary arteries are right dominant type with vessel tortuosity <30 °. The reference diameter and minimum diameter of left anterior descending coronary artery in Bama miniature pig and the Guizhou miniature pig were not significantly different (P> 0.05). The reference diameter of the right coronary artery of Bama miniature pigs was significantly smaller than that of Guizhou miniature pigs (P <0.05). There was no significant difference in the diameter of miniature pigs (P> 0.05). Conclusion In vivo and dynamic coronary angiography, it is found that adult animals in both Bama and Guizhou miniature pigs are homogenous and consistent with human. The vessel curvature is small and the catheterization is easy. The diameter of the main branch is suitable for the implantation of 2.75 Coronary stent in mm or 3.0 mm format. During chronic experimental feeding, body weight of adult animals did not change much. Therefore, both strains of miniature pigs are suitable for coronary stent evaluation study.