本文介绍作者于1983年9月至1984年3月用295例妊娠6～11周人工流产术或在B超指示下经宫颈抽吸的绒毛组织制片666份(每份组织湿重10～20mg),不经培养直接制备染色体的方法。文中详细介绍了制备方法,并对其中270份进行各项分析:发现以妊娠7～8周时分裂相出现率最高(100％)且最多,以后逐渐减少;绒毛组织用量以10～20mg为宜;秋水酰胺最终浓度和作用时间宜为0.04～0.4μg/ml,作用1小 This article describes the authors from September 1983 to March 1984 with 295 cases of pregnancy 6 to 11 weeks of abortion or under the direction of B-transvaginal villus tissue preparation 666 copies (each tissue wet weight 10 ~ 20mg ), Direct preparation of chromosome without culture. In this paper, the preparation method was introduced in detail, and 270 of them were analyzed. The highest incidence (100%) of the dividing phase was observed at 7-8 weeks gestation and then decreased gradually. The amount of villus tissue was 10-20 mg ; Colchicine final concentration and duration of action should be 0.04 ~ 0.4μg / ml, the role of a small