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目的:探讨卡托普利对糖尿病视网膜病变发生的防治作用。方法:应用免疫组织化学方法和计算机图象分析技术对糖尿病小鼠未治疗组和卡托普利治疗组视网膜组织AngII和VEGF进行免疫组织化学测定和半定量分析。结果:AngII和VEGF在两组小鼠视网膜神经节细胞和血管内皮细胞均为阳性表达,而在正常对照组两者均为阴性表达。与糖尿病未治疗组相比,卡托普利治疗组的AngII和VEGF的平均灰度值明显增加,而面密度值明显降低,说明治疗组的免疫信号降低,阳性表达细胞数减少。结论:卡托普利可降低糖尿病小鼠视网膜组织AngII和VEGF的阳性表达,推测其对糖尿病视网膜病变具有一定的保护作用。
Objective: To investigate the prevention and treatment of captopril on diabetic retinopathy. Methods: Immunohistochemistry and semi-quantitative analysis of AngII and VEGF in retina of untreated and captopril-treated diabetic mice were performed by immunohistochemistry and computer image analysis. Results: Both AngII and VEGF were positive in retinal ganglion cells and vascular endothelial cells in both groups, but negative in normal control group. Compared with untreated diabetic group, the mean gray value of AngII and VEGF in captopril treatment group increased significantly while the areal density decreased significantly, which indicated that the immune signal of treatment group decreased and the number of positive cells decreased. Conclusion: Captopril can reduce the positive expression of AngII and VEGF in the diabetic retinopathy and presumed that it has a protective effect on diabetic retinopathy.