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Aim:To assess the anti-hepatitis B virus(HBV)effect of hyperoside extractedfrom Abelmoschus manihot(L)medik.Methods:The human bepatoma HepG2.2.15 cell culture system and duck hepatitis B virus(DHBV)infection modelwere used as in vivo and in vitro models to evaluate the anti-HBV effects.Results:In the cell model,the 50% toxic concentration of hyperoside was 0.115 g/L;themaximum nontoxic concentration was 0.05 g/L,On the maximum nontoxicconcentrations,the inhibition rates of hyperoside on HBeAg and HBsAg in-the2.2.15 cells were 86.41% and 82.27% on d 8,respectively.In the DHBV infectionmodel,the DHBV-DNA levels decreased significantly in the treatment of 0.05g.kg~(-1)·d~(-1)and 0.10 g·kg~(-1)dosage groups of hyperoside(P<0.01).The inhibitionof the peak of viremia was at the maximum at the dose of 0.10 g·kg~(-1)·d~(-1)andreached 60.79% on d 10 and 69.78% on d 13,respectively.Conclusion:Theseresults suggested that hyperoside is a strong inhibitor of HBsAg and HBeAgsecretion in 2.2.15 cells and DHBV-DNA levels in the HBV-infected duck model.
Aim:To assess the anti-hepatitis B virus(HBV)effect of hyperoside extractedfrom Abelmoschus manihot(L)medik.Methods:The human bepatoma HepG2.2.15 cell culture system and duck hepatitis B virus(DHBV) infection modelwe used used in in vivo and In vitro models to evaluate the anti-HBV effects.Results:In the cell model,the 50% toxic concentration of hyperoside was 0.115 g/L;the maximumum nontoxic concentration was 0.05 g/L,On the maximum nontoxic concentration,the inhibition rates of hyperoside On HBeAg and HBsAg in-the2.2.15 cells were 86.41% and 82.27% on d 8,respectively.In the DHBV infectionmodel,the DHBV-DNA levels decreased significantly in the treatment of 0.05g.kg~(-1)·d~ (-1)and 0.10 g·kg -1 dosage groups of hyperoside (P<0.01). The inhibition of the peak of viremia was at the maximum at the dose of 0.10 g·kg -1 ·d~ (-1)andreached 60.79% on d 10 and 69.78% on d 13,respectively.Conclusion:Theseresults suggested that hyperoside is a strong inhibitor of HBsAg and HBeAgsecretion in 2.2.15 c Ells and DHBV-DNA levels in the HBV-infected duck model.