论文部分内容阅读
目的优选补肾活血颗粒的水提取工艺。方法通过单因素试验和正交试验,采用HPLC波长切换法,以杜仲的松脂醇二葡萄糖苷、红花的羟基红花黄色素A的转移率及提取液中的含量为评价指标,结合权重系数,综合考察提取方法、浸泡时间以及提取溶剂用量、提取次数、提取时间等因素对制剂提取工艺的影响。结果较优水提工艺为:12倍水量浸泡1 h,加热回流提取3次,每次1.5 h。结论 HPLC波长切换法多指标综合考察得到的补肾活血颗粒水提取工艺重复性好,工艺条件切实可行。
Objective To optimize the water extraction process of Bushen Huoxue Granule. Methods By single factor experiment and orthogonal test, HPLC wavelength switching method was used to evaluate the metastasis rate and the content of hydroxysafflor yellow A in Eucommia ulmoides Oliv. , Comprehensive study of extraction methods, soaking time and the amount of solvent extraction, extraction times, extraction time and other factors on the extraction process. Results The optimal water-extraction process was as follows: 12 times of water was soaked for 1 h, heated and refluxed for 3 times, 1.5 h each. Conclusion HPLC wavelength switching method multi-index comprehensive study of the Bushen Huoxue particles water extraction process repeatability, process conditions feasible.