论文部分内容阅读
目的研究促使人肿瘤细胞中ＴＨ２类细胞因子表达向ＴＨ０型的逆转及逆转后的肿瘤细胞对ＮＫ抗性的变化。方法首先用ＭＴＴ方法对悬浮培养的肿瘤细胞株ＤＢ大淋巴细胞瘤、Ｋａｒｐａｓ淋巴瘤、Ｍｉｃｈａｅｌ淋巴瘤、Ｒａｊｉ、ＨＬ６０和Ｋ５６２进行了ＮＫ杀伤敏感性的筛选。选择ＮＫ不敏感的Ｋａｒｐａｓ淋巴瘤和ＨＬ６０，用ｒｈＩＦＮγ、ｒｈＩＬ－１２和抗ＩＬ－１０ＭｃＡｂ经不同组合对其进行由ＴＨ２类细胞因子表达向ＴＨ１类细胞因子表达的促逆转研究，并观察促逆转后的肿瘤细胞对ＮＫ抗性的变化。结果ＲＴ－ＰＣＲ结果表明，经上述不同细胞因子组合诱导后，Ｋａｒｐａｓ淋巴瘤细胞均从表达ＴＨ２类细胞因子为主向ＴＨ０型逆转，并且各组逆转后的肿瘤细胞对ＮＫ的抗性均有不同程度的减弱。结论ＴＨ１类细胞因子（如ＩＦＮγ）、ＴＨ２类细胞因子拮抗剂（如ＩＬ－１０单抗）和ＩＬ－１２不同程度地促进肿瘤细胞表达的细胞因子由ＴＨ２型向ＴＨ０／ＴＨ１型逆转。促逆转后可以改善肿瘤细胞对机体杀伤作用的敏感性，提高抗肿瘤免疫能力
Objective To study the reversal of the expression of TH2 cytokines in human tumor cells to TH0 type and the changes in NK resistance of tumor cells after reversion. Methods The susceptibility to NK killing was evaluated by MTT assay on DB large lymphocytic tumor cells, Karpas lymphoma, Michael lymphoma, Raji, HL60 and K562 tumor cells. NK-insensitive Karpas lymphoma and HL60 were selected, and rhIFN-γ, rhIL-12, and anti-IL-10 McAb were used in different combinations to express the expression of TH2 cytokines to TH1-like cytokines, and to observe the effect of reversal. Changes in the resistance of NK cells to tumor cells. Results The results of RT-PCR showed that Karpas lymphoma cells were reversed from TH2-type cytokines to TH0-type cells after induction with the combination of the above different cytokines, and the reversal of tumor cells after NK reversal was different in each group. The degree of weakening. Conclusion TH1 cytokines (such as IFNγ), TH2 cytokine antagonists (such as IL-10 monoclonal antibody) and IL-12 cytokines that promote tumor cell expression to varying degrees are reversed from TH2 to TH0/TH1. After reversal, it can improve the sensitivity of tumor cells to killing the body and improve anti-tumor immunity.