本研究取１５～２０周人胎周围神经，放在冷冻的含ＤＭＥＭ（４０％）、ＤＭＳＯ（１０％）及小牛血清（５０％）中。细胞活性为９１％。雪旺氏细胞经过分离、培养、保持了活性。经３～６月液氮保存后，细胞活性为９４％，雪旺氏细胞在４℃下经２４小时，活性仍有９２％。从新鲜周围神经取得的和经冷冻保存的以及单个培养的雪旺氏细胞，在培养时生长缓慢，但在培养液中加牛脑垂体提取液后，细胞增殖加快。取自新鲜或冷冻的周围神经的雪旺氏细胞用Ｓ－１００蛋白抗体染色的特性相似。本文还报告了分离出的雪旺氏细胞在扫描及透射电镜下的形态特征。 In this study, peripheral human fetuses were harvested from 15 to 20 weeks in frozen DMEM (40%), DMSO (10%), and calf serum (50%). The cell activity was 91%. Schwann cells are isolated, cultured, and maintained. After 3 to 6 months of liquid nitrogen preservation, the cell activity was 94%, Schwann cells at 4 ℃ after 24 hours, the activity is still 92%. Schwann cells, taken from fresh peripheral nerves and cryopreserved, and cultured singly, grew slowly during culture, but increased after bovine pituitary extract was added to the culture. Schwann cells, taken from fresh or frozen peripheral nerves, were similarly stained with the S-100 protein antibody. This article also reports the morphological features of isolated Schwann cells under scanning and transmission electron microscopy.