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以25份克拉克和6份哈罗索近等基因系为试材,研究了播种时期,成熟期,结荚习性,抑制褐斑形成,抗病毒病及某些控制种皮颜色基因对大豆籽粒斑驳形成的影响。采用完全随机区组裂区设计。研究结果表明,播种时期对大豆的成熟期,大豆花叶病毒病及籽粒斑驳的严重度具有很大的影响。随着播期的推迟,从苗期到成熟所需要的日数明显减少,病毒病、斑驳粒率(PMS)和斑驳指数(MI)明显减轻。基因替换效应分析表明,tE1,E2,E3,Im和Rsv1对减轻籽粒斑驳症状具有显著的效应。当上述基因被其隐性等位基因替换时,斑驳粒率和斑驳指数分别减少32.1％和0.205,8.8％和0.043,6.3％和0.031,27.1％和0.212,25.3％和0.156。TE1,i—k和iR对加重籽粒斑驳的形成具有显著的效应,当这几个基因或基因组合被tel,i—i和Ir所替换时斑驳粒率和斑驳指数分别降低13.8％和0.126,28.7％和0.449,6.43％和0.115。虽然R和Dtl对大豆花叶病毒病症状表现有较大的影响,但是对籽粒斑驳的形成影响不大。
A total of 25 Clarks and 6 Hassoso and other genetic lines were used as experimental materials to study the effects of sowing date, maturity, pod habit, inhibition of brown spot formation, antiviral disease and the control of seed coat color on mottle of soybean seeds The formation of the impact. Using completely random block split design. The results showed that the sowing period had a great impact on the maturity of soybean, soybean mosaic virus disease and the severity of grain mottle. With the delay of sowing date, the number of days required from seedling to maturity decreased significantly, and the virus disease, mottled grain rate (PMS) and mottling index (MI) were significantly reduced. Genetic substitution analysis showed that tE1, E2, E3, Im and Rsv1 had significant effects on allelopathic grain mottle. When the above genes were replaced by their recessive alleles, the mottled graininess and mottling index decreased by 32.1% and 0.205, 8.8% and 0.043, 6.3% and 0.031, 27.1% and 0.212, 25.3% and 0.156, respectively. TE1, i-k and iR had a significant effect on aggravating grain mottle formation. When these genes or gene combinations were replaced by tel, i-i and Ir, the mottled graininess and mottling index decreased by 13.8% and 0.126, respectively. 28.7% and 0.449, 6.43% and 0.115 respectively. Although R and Dtl had a great influence on the symptom of soybean mosaic virus disease, it had little effect on the formation of grain mottle.