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由葡萄栽培品种“蜜汁”子房诱导的愈伤组织,经不同浓度2,4—D试验表明,0.5mg/L的2,4—D最有利于形成胚性细胞;该浓度下形成的质地紧密适度的愈伤组织经2个月的悬浮振荡培养,成功地建立了分散性好、生长旺盛的胚性细胞悬浮系;并通过培养过程中生长曲线的测定,确定了细胞悬浮系的生长特性。
Callus induced by grapevine “honey” ovary showed that 2,4-D at the concentration of 0.5mg / L was the best for the formation of embryogenic cells at different concentrations of 2,4-D, The callus of the close texture was cultivated in suspension culture after 2 months. The embryonic cell suspending system with good dispersibility and vigorous growth was successfully established. The growth curve of the cell suspension system Growth characteristics.