目的分析宫颈脱落上皮的HPV-DNA表达情况与TCT及宫颈组织病理活检之间的关系,旨在探讨出一种更为敏感与合理的宫颈病变筛查方案。方法 HPV-DNA的检测采用PCR体外扩增和DNA反向杂交相结合的DNA芯片技术,宫颈脱落细胞病变程度检测采用薄层细胞学检测系统(TCT),宫颈组织病理活检采用经典方法;将HPV-DNA表达结果与TCT结果进行阳性率分析,采用统计学方法行×列χ2检验及χ2分割检验。结果炎症组HPV-DNA阳性率分别与ASC-US组及上皮内瘤变(SIL)组的HPV-DNA阳性率相比,炎症组低于后两组,差异有统计学意义(P<0.05/3);HPV联合TCT方案组的敏感性分别与HPV方案组及TCT方案组的敏感性相比,联合组的敏感性高于后两组,差异有统计学意义(P<0.05/6)。结论宫颈癌前病变的HPV感染率高于宫颈炎症的感染率,HPV-DNA检测联合TCT检测是最有效的宫颈癌筛查手段。 Objective To analyze the relationship between HPV-DNA expression in cervical exfoliated epithelium and TCT and biopsy of cervical tissue in order to explore a more sensitive and reasonable cervical lesions screening program. Methods The detection of HPV-DNA was carried out by DNA microarray combined with PCR in vitro amplification and reverse hybridization. The degree of cervical exfoliated cytopathy was detected by TLCT and the cervical biopsy was performed by classical method. -DNA expression results and TCT results for positive rate analysis, the use of statistical methods × × χ2 test and χ2 test. Results The positive rate of HPV-DNA in inflammation group was lower than that in ASC-US group and SIL group (P <0.05), and the difference was statistically significant 3). The sensitivities of HPV combined with TCT regimen group were higher than that of HPV regimen group and TCT regimen group, respectively. The sensitivity of combination group was higher than that of the latter two groups (P <0.05 / 6). Conclusion HPV infection rate of cervical precancerous lesions is higher than infection rate of cervical inflammation. HPV-DNA detection combined with TCT detection is the most effective cervical cancer screening method.