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为探讨中药越橘总黄酮是否具有抗菌、抗病毒作用,我们对越橘的乙醇提取物一总黄酮进行了体外抗菌实验和抗病毒的动物实验研究,现将研究结果报告如下: 材料与方法 1.药物:越橘全草的乙醇提取物——越橘总黄酮,该药为淡黄色粉末,1克纯品相当生药200克,其LD_(50)为1.673g/kg。 2.菌株:从卫生部药品生物制品检定所购买的标准菌株。 3.病毒:为哈尔滨医科大学公卫学院刘瑞璋教授驯化的引起小病鼠肺炎的流行性感冒病毒(A_1)株。 4.动物:由哈尔滨医科大学公卫学院动物室提供的昆明系健康杂交小白鼠。 5.方法: (1)抗菌实验:
In order to investigate whether the Chinese bilberry total flavonoids have antibacterial and antiviral effects, we conducted in vitro antibacterial experiments and antiviral animal experiments on the biloba extract-total flavonoids. The results of the study are reported as follows: Materials and Methods 1 Drugs: Ethanol extract of bilberry whole plant - bilberry total flavonoids, the drug is light yellow powder, 1 gram of pure product is quite crude drug 200 grams, its LD_ (50) is 1.673g/kg. 2. Strains: standard strains purchased from the Ministry of Health’s Pharmaceutical and Biological Products Verification. 3. Virus: Influenza virus (A_1) strain that causes domestic rat pneumonia caused by Professor Liu Ruizhen of the School of Public Health, Harbin Medical University. 4. Animals: Kunming healthy hybrid mice provided by the animal room of Harbin Medical University School of Public Health. 5. Methods: (1) Antibacterial experiment: