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本文给出了应用膜上斑点免疫吸咐试验(Dot—ELISA)检测黑尾叶蝉体内水稻黄矮病毒的方法,试验研究表明:使用该方法检测比ELISA法不仅操作简便、使用抗原少,而且快速、灵敏度更高并可长期保存结果。因此,作者认为该法可作为基层单位测报时应用。
In this paper, the method of Dot-ELISA to detect rice yellow dwarf virus in rice leafhopper is presented. The experimental results show that this method not only has the advantages of simple operation, less antigens, moreover, Fast, sensitive and save results in the long term. Therefore, the author believes that the law can be used as a unit of measurement when the grass-roots units.