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目的 利用基因工程技术构建人骨形态发生蛋白 2重组腺病毒 ,并进行体内表达 ,测定活性 ,为深入开展的基因治疗研究创造条件。方法 同源重组构建重组腺病毒 ,将 30只SD大鼠随机分为治疗组和对照组 ,将纯化后的重组腺病毒 5 0 μl注入SD大鼠股部肌肉陷窝病损处 ,通过组织学染色、X线片对动物模型的组织变化进行观测。结果 重组腺病毒治疗组有明显的诱导成骨活性 ,治疗组碱性磷酸酶活性明显高于对照组。结论 应用于动物实验中目的基因表达 ,并具有生物学活性。本研究是在骨缺损基因治疗方面的一次尝试 ,且人骨形态发生蛋白 2重组腺病毒的构建成功 ,也为我国骨科疾病的基因治疗进一步研究奠定了基础。
OBJECTIVE: To construct human bone morphogenetic protein-2 recombinant adenovirus using gene engineering and to express in vitro and in vivo, so as to create conditions for gene therapy research in depth. Methods Homologous recombination was used to construct recombinant adenovirus. Thirty SD rats were randomly divided into treatment group and control group. Fifty microliters of the purified recombinant adenovirus was injected into the muscle lacunar defect of SD rats. Dyeing, X-ray observation of the tissue changes in animal models. Results The recombinant adenovirus treatment group had obvious osteogenic activity, and the activity of alkaline phosphatase in the treatment group was significantly higher than that in the control group. Conclusions It is applied to the expression of target genes in animal experiments and has biological activity. This study is an attempt to gene therapy of bone defects, and the successful construction of human bone morphogenetic protein 2 recombinant adenovirus laid a foundation for further study of gene therapy for orthopedic diseases in China.