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目的：观测β－葡萄糖醛酸酶（β－Ｇｌｕｃｕｒｏｎｉｄａｓｅ，β－Ｇ）在人体胰腺导管上皮细胞与胰腺癌细胞中的定位，探索良、恶两种细胞内β－Ｇ的差异。方法：采用低温包埋，紫外线照射，超薄切片，胶体金标记，免疫电镜方法。结果：β－Ｇ定位于内质网和溶酶体内，同时观察到胰腺癌细胞中标记β－Ｇ的金颗粒数目明显多于胰腺导管上皮细胞中金颗粒数目。结论：癌细胞分泌β－Ｇ增多，对胰腺癌的早期发现、早期诊断有一定意义。
Objective: To observe the localization of β-glucuronidase (β-G) in human pancreatic ductal epithelial cells and pancreatic cancer cells, and to explore the difference of β-G between benign and malignant cells. Methods: Low temperature embedding, ultraviolet radiation, ultrathin section, colloidal gold labeling, and immunoelectron microscopy. RESULTS: β-G was localized in endoplasmic reticulum and lysosomes, and the number of gold particles labeled with β-G in pancreatic cancer cells was significantly greater than that in pancreatic ductal epithelial cells. Conclusion: The increased secretion of β-G in cancer cells has certain significance for the early detection and early diagnosis of pancreatic cancer.