论文部分内容阅读
[Objective]To determine the content of rutin and quercetin in the whole plant of Yantai Bupleurum by HPLC. [Methods] An Agilent C18column( 250 mm × 4. 6 mm,5 μm) was applied with a mobile phase of acetonitrile- 0. 1% phosphoric acid solution,gradient elution was selected with the flow rate of 1. 0 mL / min,the UV detector was set at 360 nm. [Results]The average recovery rates of rutin and quercetin were 100. 47% and 99. 50%,the RSD were 1. 34% and 0. 41%. The average content of rutin and quercetin in the whole plant of Yantai Bupleurum reached 83. 00 and 102. 68 μg / g. [Conclusions]The method is fast,accurate and reliable. It is suitable for content determination of rutin and quercetin in the whole plant of Yantai Bupleurum.
[Objective] To determine the content of rutin and quercetin in the whole plant of Yantai Bupleurum by HPLC. [Methods] An Agilent C18column (250 mm × 4.6 mm, 5 μm) was applied with a mobile phase of acetonitrile-0. 1% phosphoric acid solution, gradient elution was selected with the flow rate of 1. 0 mL / min, the UV detector was set at 360 nm. [Results] The average recovery rates of rutin and quercetin were 100. 47% and 99. 50%, the RSD were 1. 34% and 0. 41%. The average content of rutin and quercetin in the whole plant of Yantai Bupleurum reached 83.00 and 102. 68 μg / g. [Conclusions] The method is fast, accurate and reliable. It is suitable for content determination of rutin and quercetin in the whole plant of Yantai Bupleurum.