论文部分内容阅读
目的 观察脂多糖(lipopolysaccharide , LPS)对大鼠黑质纹状体系统的神经毒性作用,探讨帕金森病(Parkinson disease,PD)与炎症反应的关系。方法 采用立体定向术向大鼠脑黑质注射5μg LPS,于注射后不同时间点(1、3、7、14、21、30 d)观察经腹腔注射阿朴吗啡后大鼠的旋转行为以及采用高效液相色谱(HPLC)测定黑质 纹状体单胺类递质含量的变化;通过免疫组化观察多巴胺(dopamine,DA)合成限速酶酪氨酸羟化酶(tyro sine hydroxylase,TH)的变化。结果 LPS注射后 14、21、30 d大鼠出现向注射侧的旋转行为,黑质 纹状体 DA及其代谢产物随时间延长呈不同程度下降(P<0 05),而 5 羟色胺(5 HT)仅有短暂下降,去甲肾上腺素(nora drenaline,NA)无变化。TH阳性细胞数在第3天开始下降为对照组的45%,14 d时为对照组的5%~10%,30 d时几乎完全消失。结论 LPS注射黑质后能特异性损害DA能神经元,可用于建立PD免疫炎症动物模型。
Objective To investigate the neurotoxic effect of lipopolysaccharide (LPS) on nigrostriatal system in rats and to explore the relationship between Parkinson disease (PD) and inflammatory reaction. Methods Stereotactic technique was used to inject 5 μg of LPS into the substantia nigra of rats. The rotation behavior of rats after ip injection of apomorphine was observed at different time points (1, 3, 7, 14, 21 and 30 d after injection) The content of monoamine neurotransmitters in nigrostriatal system was measured by high performance liquid chromatography (HPLC). The expression of tyrosine hydroxylase (TH), a rate-limiting enzyme in dopamine (DA) The change. Results The rats were injected with LPS for 14, 21, 30 days after injection. The rotation of the striatum occurred on the injection side. The DA and its metabolites of nigrostriatal decreased with time (P <0.05), while the serotonin (5 HT ) Only a brief decline, noradrenaline (nora drenaline, NA) did not change. The number of TH positive cells decreased to 45% of the control group on the third day, from 5% to 10% of the control group on the 14th day, almost disappeared on the 30th day. Conclusions LPS injected into substantia nigra can specifically damage DA neurons and can be used to establish animal model of PD immune inflammation.