为了了解分泌性中耳炎与衣原体的关系,文中报告用多聚酶链反应(PCR)法对31例分泌性中耳炎患者的中耳渗液进行肺炎衣原体检测,6例呈阳性反应,阳性率为19.3%,通过对照可排除假阴性和假阳性.用PCR法检测中耳渗液中沛炎衣原体简便、快速、待异性高、敏感性强,优于其他检测方法. In order to understand the relationship between secretory otitis media and chlamydia, the paper reported the detection of Chlamydia pneumoniae in 31 cases of secretory otitis media by polymerase chain reaction (PCR), 6 cases were positive, the positive rate was 19.3% Control can be ruled out false negative and false positive. PCR detection of middle ear exudate Chlamydia pneumoniae is simple, fast, to be heterosexual, sensitive, superior to other detection methods.