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目的:探讨慢性丙型肝炎病毒(HCV)感染患者丙型肝炎病毒RNA(HCV RNA)增殖状态与T细胞亚群功能及血浆中IL-2和slL-2R活性的关系。方法:以间接免疫荧光法,ELISA法和RT-PCR分别检测75名慢性HCV感染患者(其中HCV RNA阳性47例,HCV RNA阴性28例)外周血T细胞亚群,IL-2及sIL-2R的水平和HCV RNA。结果:慢性HCV感染患者周围血CD3~+,CD4~+淋巴细胞亚群,CD4~+/CD8~+比值及IL-2水平均显著低于正常对照组(P<0.01),而sIL-2R明显升高(P<0.01);血清HCV RNA阳性患者T细胞亚群,CD4~+/CD8~+比值及IL-2水平显著低于HCV RNA阴性患者(p<0.01)。结论:慢性HCV感染患者的机体免疫功能紊乱。细胞免疫功能低下,HCV RNA阳性患者较阴性患者更甚,提示细胞免疫功能受抑可能是HCV持续增殖的原因。
Objective: To investigate the relationship between the proliferation of hepatitis C virus RNA (HCV) and the function of T lymphocyte subsets and the activity of IL-2 and SLL-2R in plasma of patients with chronic hepatitis C virus (HCV) infection. Methods: T-cell subsets, IL-2 and sIL-2R in peripheral blood of 75 patients with chronic HCV infection (including 47 HCV RNA positive and 28 HCV RNA negative) were detected by indirect immunofluorescence, ELISA and RT- Level and HCV RNA. Results: The levels of CD3 ~ +, CD4 ~ + lymphocyte subsets, CD4 ~ + / CD8 ~ + ratio and IL-2 in peripheral blood of patients with chronic HCV infection were significantly lower than those in the normal control (P <0.01) (P <0.01). The levels of T cell subsets, CD4 ~ + / CD8 ~ + ratio and IL-2 in patients with positive HCV RNA were significantly lower than those in HCV RNA negative patients (p <0.01). Conclusion: The immune function of patients with chronic HCV infection is disturbed. Cellular immune dysfunction, HCV RNA positive patients even more than negative patients, suggesting that suppression of cellular immune function may be the reason HCV continues to proliferate.