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目的评价云南鼠疫菌pYC质粒标识基因的特异性及在鼠疫监测中的应用。方法应用pYC质粒标识基因引物yd1,yd2并以鼠疫菌特异性基因pla,fra作为对照进行PCR实验,观察实验室感染标本和现场样品的检出情况。结果共检测实验感染动物脏器8份,均为阳性。检测现场收集的黄胸鼠脏器22份,阳性6份,阳性率27.27%。检测感染的蚤类67份,阳性13份,阳性率19.40%,试验引物yd1,yd2与对照引物pla,fraPCR检测结果其特异性和敏感性均一致。结论将yd1,yd2引物,联合pla,fra引物,建立鼠疫PCR快速诊断方法,应用于云南、广西、贵州判断鼠疫菌、监测鼠疫信息,具有重要的实际意义。
Objective To evaluate the specificity of pYC plasmid marker gene in Y. pestis and its application in the surveillance of plague. Methods The primers yd1 and yd2 of pYC plasmids were used to identify plastids, and pla and fra were used as controls to detect PCR samples and laboratory samples. Results A total of 8 experimental animals infected with animal were tested positive. Twenty-two organs of the labiathera collected from the test sites were positive, with a positive rate of 27.27%. 67 were tested for fleas, 13 were positive, the positive rate was 19.40%. The specificity and sensitivity of test primers yd1 and yd2 were the same as those of pla and fraPCR. Conclusion It is of great practical significance to establish a rapid diagnostic method for plague PCR using yd1 and yd2 primers in combination with pla and fra primers in Yunnan, Guangxi and Guizhou provinces to determine plague pathogens.