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目的 观察坐浴二号洗液对小鼠耳廓肿胀炎症模型和豚鼠磷酸组胺致痒模型的抗炎、止痒作用.方法 将雄性昆明小鼠50只和豚鼠50只,分别随机分为空白对照组、阳性对照组和坐浴二号洗液低剂量组、中剂量组、高剂量组,每组10只.昆明小鼠每天分别涂抹蒸馏水、金玄痔科熏洗散和低、中、高剂量坐浴二号洗液于右耳廓左右两面2次,连续给药6d;豚鼠右后脚背脱毛,每天分别涂抹蒸馏水、赛庚啶软膏和低、中、高剂量坐浴二号洗液2次.末次给药30 min后,小鼠于右耳廓两面涂二甲苯致肿,检测各组耳廊肿胀度;豚鼠用砂布擦伤豚鼠右后脚背脱毛处,在创面滴磷酸组胺致痒,以豚鼠出现回头舔后足时所给予的磷酸组胺总量为致痒阈,记录并比较各组的致瘁阈.结果 至用药结束,各组均未见死亡.高剂量纽、中剂量组、低剂量组、阳性对照组都能够明显降低小鼠耳廓肿胀度和提高豚鼠的致痒阈,与空白组比较有显著性差异(P＜0.05,P＜0.01).其中高剂量组和中剂量组小鼠耳廓肿胀度降低,与阳性对照组比较差异有统计学意义(P＜0.01);中剂量组和低剂量组豚鼠致痒阈明显低于阳性对照组,差异有统计学意义(P＜0.01).结论 坐浴二号洗液有明显的抗炎和止痒作用,且其药效作用随药物浓度升高而增强.“,”Objective To observe the effect of anti-inflammatory and anti-pruritic of Zuoyu No.2 washing lotion on mouse ear edema model and guinea pig histamine phosphate induced itching model.Methods 50 male Kunming mice and 50 guinea pigs were randomly divided into control group,positive control group,low dose,middle dose and high dose Zuoyu No.2 washing lotion groups,10 mice in each group.Kunming mice were treated with distilled water,Jinxuan Zhike fumigating and Zuoyu No.2 washing lotion on both sides of the right ear,2 times every day,continuous administration of 6d.Right after the instep hair removal respectively,every guinea pig was smeared with distilled water,cyproheptadine ointment and low,middle and high dose Zuoyu No.2 washing lotion,30 min after the last administration,the mice in the right auricle swelling induced by xylene were coated on both sides,to observe the ear swelling degree.Groups of guinea pigs were scratched with emery cloth right after hair removal at the instep,histamine phosphate in total for the back foot licking itch threshold,to observe and record the itch threshold.Results No death was found in all groups until the end of the drug.High dose group,middle dose group,low dose group,positive control group could significantly reduce the swelling of auricle of mice and improve the itch threshold of guinea pigs,there were significant differences compared with the control group (P＜0.05);the swelling of ear in mice reduced in high dose group and middle dose group,compared with positive control group,there was statistically significant difference (P＜0.01);itch valve in middle dose group and low dose group was significantly lower than positive control group,the difference was statistically significant (P＜0.01).Conclusion Zuoyu No.2 washing lotion has obvious anti-inflammatory and antipruritic effect,and the effect of the drug was enhanced with the increase of drug concentration.