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海参毒B(Holothurine B)来源于热带浅海中黑海参(Holothuria atra))。将此海洋动物的体壁肌肉的水醇提取物,经真空浓缩,残渣溶于水,先用己烷提取后,再用正丁醇提取数次,蒸干正丁醇液,残渣溶于热乙醇中,用活性炭处理半小时,过滤,滤液冷却至室温,得微细结晶性沉淀,乙醇重结晶四次,即获得层析纯的海参素B(展开剂氯仿-甲醇-水=60:30:1;吸附剂KSK硅胶;显色剂硫酸),为无色针状结晶,熔点216～218°[α]_D~(25)-33°(c=1.25,吡啶)。经一系列化学和物理化学方法确定,海参素B在糖键上含有一个磺酸基,结构式为:
Holothurine B is derived from Holothuria atra in tropical shallow sea). The marine animal body wall muscle hydroalcoholic extract, concentrated in vacuo, the residue dissolved in water, first extracted with hexane, and then extracted with n-butanol several times, evaporated to dry n-butanol solution, the residue dissolved in heat Ethanol, treated with activated carbon for half an hour, filtered, the filtrate was cooled to room temperature to obtain a fine crystalline precipitate, ethanol recrystallized four times to obtain chromatographic pure sea cucumber B (developing solvent chloroform - methanol - water = 60:30: 1; adsorbent KSK silica gel; developer sulfuric acid) as colorless needles, m.p. 216-218 ° [α] -D ~ (25) -33 ° (c = 1.25, pyridine). After a series of chemical and physical and chemical methods to determine, sea cucumber B in the sugar bond contains a sulfonic acid group, the formula is: