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目的：阐明硒（Ｓｅ）、维生素Ｅ（ＶＥ）与心肌胶原蛋白代谢的关系。方法：以病区粮（低Ｓｅ、低ＶＥ）喂养大鼠，并注射异丙基肾上腺素（ＩＳＯ）诱发心肌缺血缺氧性坏死，检测心肌中谷胱甘肽氧化酶（ＧＳＨ－ＰＸ）、过氧化氢酶（ＣＡＴ）、丙二醛（ＭＤＡ）和胶原蛋白含量变化。结果：低Ｓｅ低ＶＥ组血浆和心肌ＧＳＨ－ＰＸ、ＣＡＴ活性下降，ＭＤＡ含量上升，与心肌胶原蛋白含量变化有相关性。补Ｓｅ补ＶＥ后，ＧＳＨ－ＰＸ、ＣＡＴ活性上升，ＭＤＡ水平下降，心肌胶原蛋白含量下降。结论：低Ｓｅ低ＶＥ加重ＩＳＯ诱发的心肌损伤，脂质过氧化和自由基与心肌胶原蛋白代谢关系密切。补Ｓｅ补ＶＥ后，能缓解心肌细胞对诱发因素所造成的损伤，对保护心肌细胞和防止心肌纤维化的形成具有一定作用。
Objective: To clarify the relationship between selenium (Se), vitamin E (VE) and myocardial collagen metabolism. Methods: Wistar rats were fed with low Se and low VE, and induced by ISO. The levels of glutathione peroxidase (GSH-PX) in myocardium were measured. Catalase (CAT), malondialdehyde (MDA) and collagen content. Results: The activity of GSH-PX and CAT in plasma and myocardium of low-Se-low VE group decreased and the content of MDA increased, which was correlated with the change of myocardial collagen content. After supplementing Se with VE, GSH-PX, CAT activity increased, MDA level decreased, myocardial collagen content decreased. CONCLUSION: Low Se and low VE aggravate ISO-induced myocardial injury. Lipid peroxidation and free radicals are closely related to myocardial collagen metabolism. Se make up VE, can alleviate the damage caused by the cardiomyocytes induced factors, to protect the myocardial cells and prevent the formation of myocardial fibrosis have a certain role.