显示酸性及碱性磷酸酶的方法很多。但是经典的金属盐沉淀法由于受到多方面因素的影响可以出现假阴性、假阳性以及错误的定位,较理想的还是用偶氮法。然而偶氮法由于底质及重氮盐的不同,反应结果也不一致。萘酚AS磷酸酯是一种取代萘酚(substituted naphthols)。这种底质在合适的PH中经磷 There are many ways to show acidity and alkaline phosphatase. However, due to the influence of many factors, the classical metal salt precipitation method can be false negative, false positive and wrong positioning, and the azo method is more desirable. However, the azo method due to different substrate and diazonium salt, the reaction results are inconsistent. Naphthol AS phosphate is a substituted naphthols. This substrate is phosphated in a suitable pH