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为了方便准确评价麦田捕食性天敌对麦长管蚜Sitobion avenae(Fabricius)的控制作用,应用单克隆抗体及间接ELISA方法研究了捕食量和温度对麦长管蚜在多异瓢虫Adonia variegata(Goeze)体内可测定时间的影响。结果表明:在不同的温度和捕食量下,麦长管蚜在多异瓢虫体内的降解中间产物曲线均为单峰型。温度对猎物的可测定时间有显著的影响,随着温度的上升,猎物的可测定时间不断缩短。特别是当温度达到30℃时,猎物的降解速率迅速上升,其可测定时间只有1.18天。捕食量对猎物的可测定时间也有显著影响,随着捕食量的增加,猎物的可测定时间延长。在25℃的条件下,当捕食量从1头蚜虫增加到3头蚜虫,可测定时间从2.81天延长到4.25天。
In order to facilitate the accurate and accurate evaluation of the control effect of predatory natural enemies on the Sitobion avenae (Fabricius) in wheat field, the effects of predation and temperature on the growth of S. avenae in Adonia variegata (Goeze ) The impact of time can be measured in vivo. The results showed that under different temperatures and prey, the midgut degradation curve of Aphis gossypii was unimodal. Temperature can have a significant effect on the measurable time of the prey, as the temperature rises, the pre-measurable time of the prey is continuously shortened. Especially when the temperature reaches 30 ℃, the rate of prey degradation rapidly increases, and its measurable time is only 1.18 days. Predation of prey can also have a significant impact on the determination of time, with the increase of prey, prey measurable time extension. At 25 ° C, measurable time increased from 2.81 days to 4.25 days when the predation rate increased from 1 aphid to 3 aphids.