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受枸杞道地产区土地资源等因素限制,连作障碍已成为影响枸杞产业发展的重要原因之一,导致严重的经济损失。研究连作条件下枸杞农田土壤生态系统微生物群落的演替规律对枸杞产业的可持续发展具有重要的理论意义。以宁夏银川市南梁农场连作多年的枸杞地为研究对象,利用Illumina Mi Seq测序技术分析了连作对再植枸杞根际/非根际细菌群落的影响。结果表明,连作地显著抑制再植枸杞苗胸径的增加,且其土壤p H较对照样地显著降低(p<0.05)。测序结果证实,与对照样地相比,连作地再植枸杞根际土壤细菌物种数显著降低(p<0.05),细菌群落α多样性下降(p>0.05)。主坐标分析表明,连作和对照样地间枸杞非根际细菌群落结构无明显差异,但连作显著改变再植枸杞根际细菌的群落结构。对细菌群落丰度的统计分析发现,连作地枸杞根际浮霉菌门、非根际假单胞菌门的相对丰度较对照样地显著降低(p<0.05)。此外,冗余分析结果表明:枸杞园土壤p H和有效磷含量是影响枸杞非根际土壤细菌群落结构变化的主要因素,分别解释了41.8%和35.4%的群落结构变化(p<0.05),其他土壤因子无统计学意义,但土壤理化因子对再植枸杞根际细菌群落结构变化的影响均未达显著水平。这些结果证实连作能够显著抑制再植枸杞生长、影响再植枸杞根际细菌群落结构和多样性,干扰枸杞与土壤细菌群落间的互作关系。这些研究结果将为解析枸杞连作障碍机制提供理论基础。
Owing to the limited land resources and other factors in the real estate area of wolfberry road, continuous cropping obstacle has become one of the important reasons that affect the development of wolfberry industry, resulting in serious economic losses. It is of great theoretical significance to study the succession of microbial communities in the soil ecosystem of Lycium barbarum under continuous cropping conditions for the sustainable development of the Chinese wolfberry industry. In this paper, we studied the effects of continuous cropping on rhizosphere and non-rhizosphere bacterial communities in replanted Lycium barbarum L. with Illumina Mi Seq sequencing technology. The results showed that, in continuous cropping, the DBH diameter of replanted goji was significantly inhibited, and the soil p H was significantly lower than that of the control (p <0.05). Sequencing results showed that compared with the control plots, the number of bacterial species in the rhizosphere soil of replanted Lycium barbarum decreased significantly (p <0.05) and the α diversity of bacterial communities decreased (p> 0.05). The main coordinate analysis showed that there was no significant difference in the non-rhizosphere bacterial community structure between the continuous cropping and the control plot, but the continuous cropping significantly changed the community structure of rhizosphere bacteria in the replanted goji. The statistical analysis of the abundance of bacterial communities showed that the relative abundance of the non-rhizosphere bacteria and non-rhizosphere Pseudomonas sp. Decreased significantly (p <0.05) compared with the control. In addition, the results of redundancy analysis showed that p H and available P content in the soil of Lycium barbarum were the main factors affecting the community structure of the soil non-rhizosphere soil, and explained 41.8% and 35.4% of the community structure changes (p <0.05) Other soil factors were not statistically significant, but soil physical and chemical factors had no significant effect on the changes of bacterial community structure in the rhizosphere of replanted Lycium barbarum. These results confirmed that continuous cropping could significantly inhibit the growth of replanted Goji berry, the rhizosphere bacterial community structure and diversity of replanted Goji berry, and the interference of the interaction between Wolfberry and soil bacterial communities. These findings will provide a theoretical basis for understanding the mechanism of continuous cropping obstacles in Chinese wolfberry.