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从1984年10月开始,作者以胎胰为供体组织,用组织培养方法制备移植物,选用受体大网膜包裹胎胰胰岛组织,进行了胰岛移植治疗大鼠链脲霉素糖尿病的实验研究。实验动物为封闭群Wistar大鼠,链脲霉素按80mg/kg的剂量经后肢肌肉1次注入。供体胎胰组织的制备:成年大鼠分笼交配,以观察到阴栓的次日作为雌鼠妊娠的第一天;从妊娠17~18天的孕鼠子宫中无菌取出胎鼠,切取胎胰。实验分3组,Ⅰ组:糖
From October 1984 onwards, the author of fetal pancreatic donor tissue, tissue culture method for the preparation of the graft, the recipient omentum wrapped fetal pancreatic islets, the islet transplantation for the treatment of streptozotocin in rats with diabetes experiment the study. The experimental animal was a closed group of Wistar rats, and streptozotocin was infused via the hindlimb muscles once at a dose of 80 mg / kg. Preparation of donor fetal pancreatic tissue: Adult rats were sub-caged mating to observe the day after the vaginal suppository as the first day of female pregnancy; fetus was removed aseptically from pregnant uterus of pregnant rats from 17 to 18 days of gestation, Fetal pancreas The experiment was divided into 3 groups, group Ⅰ: sugar