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应用显微准弹性激光散射（ＭＱＬＳ）技术与显微生物医学图象分析技术对地中海贫血红细胞及胞内血红蛋白动态特性进行了研究．在实验中，比较了正常人及地贫患者红细胞胞内血红蛋白聚集体的平均流体力学半径、平均平动扩散系数及红细胞膜的搏动频率等动态特性参数，以及细胞的截面积、规化形状因子、长径、短径、灰度等图象分析数据，发现地贫红细胞的血红蛋白聚合物平均流体力学半径远远大于正常人红细胞的，其大小变异亦较正常人大，且其膜搏动频率也较为缓慢，细胞的截面积也变小．这反映了地贫红细胞内有较大的蛋白质聚合物存在和红细胞变形能力差的特性．研究还表明，显微准弹性激光散射技术结合图象分析技术，可使测量的可比性和准确性大大提高，预期可广泛适用于各种活细胞动态特性的研究
The dynamic characteristics of thalassemia erythrocytes and intracellular hemoglobin were studied by using microphysical elastic laser light scattering (MQLS) and microscopic medical image analysis techniques. In the experiment, we compared the dynamic parameters such as average hydrodynamic radius, average translational diffusion coefficient and pulsatile frequency of erythrocyte membrane in normal and thalassemia patients, as well as the cell cross-sectional area, normalized shape factor , Long diameter, short diameter, gray scale and other image analysis data found that thalassemia erythrocyte hemoglobin polymer average hydrodynamic radius is much larger than normal human erythrocyte, its size variation than normal people, and its membrane pulsation frequency is also more Slowly, the cross-sectional area of cells also becomes smaller. This reflects the presence of larger protein polymers in thalassemic erythrocytes with poor erythrocyte deformability. The study also shows that the microscopic quasi-elastic laser scattering technology combined with image analysis technology can greatly improve the comparability and accuracy of measurements and is expected to be widely applicable to the study of the dynamic characteristics of various living cells