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1997～ 2 0 0 0年 ,用含 Bt毒蛋白 (Cry1 Ac)的人工饲料对来自本所试验棉田的棉铃虫经过室内2 1代中 1 6代次的筛选 ,筛选后 F19代 L C50 值(4.3 6 4 6 g· L- 1)比筛选前 F2 代 L C50 值 (0 .2 972 g·L- 1)提高了 1 4.7倍。试验还发现雌性棉铃虫对Bt毒蛋白的敏感性大于雄性。对 Bt毒蛋白抗性品系筛选前后分别测定了其不同菌系 (商品制剂Dipel和 Xentari)的剂量 -死亡率回归线 ,发现Bt毒蛋白抗性品系与其不同菌系间不存在明显的交互抗性
From 1997 to 2000, the cotton bollworm from cotton field in this study was screened by 16 generations of 21 generations in the laboratory with artificial feed containing Bt toxin (Cry1Ac) 4.3 6 4 6 g · L -1) was 14.7 times higher than the L C50 F2 generation before selection (0.2272 g · L -1). The test also found that female cotton bollworm Bt toxic protein sensitivity than male. The dose-mortality regression curves of different bacterial strains (commercial preparations Dipel and Xentari) were determined before and after the Bt virulence-resistant strains were screened, and it was found that there was no significant interaction resistance between the Bt virulence resistant strains and their different strains