论文部分内容阅读
目的初步研究甘草酸(Glycyrrhizic acid,GA)对氧化损伤小肠上皮细胞IEC-6的保护作用及可能机制。方法实验设空白对照组,H2O2模型组,GA低、中、高剂量组(50,100,200μg/mL)。GA各组加入相应浓度的药物作用48 h后,与模型组一起加入200μmol/L的H2O2作用2 h,空白对照组正常培养。用单细胞凝胶电泳检测DNA损伤,RT-PCR检测p53,Gadd45α mRNA表达,Western blot检测p53,Gadd45α蛋白表达。结果与空白对照组比较,模型组DNA损伤严重,Gadd45α mRNA和蛋白表达增高;与H2O2模型组比较,GA各组DNA损伤明显减轻,GA100μg/mL和200μg/mL组p53 mRNA及其蛋白表达明显升高,同时Gadd45α mRNA及其蛋白表达明显升高。结论 GA在100～200μg/mL可以保护H2O2诱导的小肠上皮细胞DNA损伤,其机制可能与p53和Gaad45α的表达增高相关。
Objective To study the protective effect and possible mechanism of Glycyrrhizic acid (GA) on IEC-6 induced oxidative damage of intestinal epithelial cells. Methods The experiment set blank control group, H2O2 model group, GA low, medium and high dose group (50,100,200μg / mL). GA groups were added with corresponding concentration of drug for 48 h, 200 μmol / L H2O2 was added to the model group for 2 h, and the blank control group was cultured normally. The DNA damage was detected by single cell gel electrophoresis. The expression of p53 and Gadd45α mRNA was detected by RT-PCR. The expression of p53 and Gadd45α protein was detected by Western blot. Results Compared with the blank control group, DNA damage of the model group was severe and the expression of Gadd45α mRNA and protein was increased. Compared with the H2O2 model group, the DNA damage of GA group was significantly reduced. The expression of p53 mRNA and protein in GA100μg / mL and 200μg / mL groups was significantly increased High, while Gadd45α mRNA and protein expression was significantly increased. Conclusions GA at 100 ~ 200μg / mL can protect H2O2-induced intestinal epithelial cells from DNA damage, which may be related to the increased expression of p53 and Gaad45α.