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建立了蔗糖脂肪酸酯中一酯～三酯的薄层分离定性及薄层扫描定量方法。以氯仿 甲醇 醋酸 水 (体积比为 80∶1 0∶8∶1 )为展开剂 ,双波长反射法锯齿扫描 ,测定波长为 530nm ,参比波长为 70 0nm ,线性范围为 4μg～60 μg ,相关系数为0 9940～ 0 9980 ,平均回收率为 96 45%～ 98 73% (RSD为 2 8%～ 3 3% ,n =3)。方法可靠 ,数据准确 ,操作简便易行 ,线性范围宽。
A qualitative and TLC scanning method for the determination of monoesters and triesters of sucrose fatty acid esters was established. Chloroform-methanol-acetic acid water (volume ratio of 80: 1 0: 8: 1) as the developing agent, dual-wavelength reflectometry sawtooth scan, the determination of wavelength 530nm, the reference wavelength of 70 0nm, linear range of 4μg ~ 60μg, The coefficients ranged from 0,940 to 0,980, with an average recovery of 96 45% to 98 73% (RSD 28% to 33%, n = 3). Method is reliable, accurate data, easy to operate, linear range.