采用Ni2 +亲和层析方法 ,对用工程化大肠杆菌BL2 1(DE3)表达的重组鼠疫菌V抗原进行纯化 ,目标蛋白纯度达到 90 %以上。以氢氧化铝凝胶配制吸附疫苗 ,经二针次肌内注射免疫实验豚鼠后 ,对皮下注射 4 0 0个致死剂量 (MLD)强毒鼠疫菌攻击有一定保护效力 ,存活率为 2 0 %。结果表明 ,重组鼠疫菌V抗原有望作为改进的F1+V亚单位疫苗的主要成分 Recombinant Yersinia pestis V antigen expressed in the engineered E. coli BL21 (DE3) was purified by Ni2 + affinity chromatography and the target protein purity was over 90%. The aluminum hydroxide gel was used to prepare the adsorbed vaccine. After intramuscular injection of guinea pig with intraperitoneal injection of two doses and several doses of guinea pigs, the protective effect against subcutaneous injection of 400 lethal dose (MLD) virulent plague bacteria was achieved with a survival rate of 20% . The results show that the recombinant Yersinia pestis V antigen is expected to be the main component of the improved F1 + V subunit vaccine