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目的：阐明ｃｍ ｙｃ 表达在肾癌发生、发展中的作用及临床意义。方法：利用免疫组织化学 Ａ Ｂ Ｃ 法结合微波抗原修复和流式细胞技术，对ｃｍ ｙｃ 蛋白在 ５２ 例肾癌组织及 １４ 例癌旁正常肾组织中的表达进行了定性、定量的测定。结果：肾癌组织中ｃｍ ｙｃ 蛋白的阳性率和阳性细胞率分别为７６．９２％ 和６５．４５％ ±２９．１９％ ，高于正常肾组织的１４．２９％ 和８．５９％ ±３．６６％ （ Ｐ ＜ ０．００５）。ｃｍ ｙｃ在肾癌组织中５５．７７％ 分布于胞浆，２１．１５％ 分布于胞核，而在正常肾组织则全部分布于胞核。其胞浆表达在病理 Ｇ２、 Ｇ３ 级高于 Ｇ１ 级（χ２＝ ９．２４４， Ｐ ＜ ０．００５）；临床分期 Ｔ３、 Ｔ４ 期高于 Ｔ１、 Ｔ２ 期（ Ｐ＜ ０．００１）。结论：ｃｍ ｙｃ 的胞浆表达促进肾癌的发生、发展，并与恶性程度、临床分期有关，是预后不良的重要指征。
Objective: To clarify the expression of c y yc in the occurrence and development of renal cell carcinoma and its clinical significance. Methods: The expression of c-yc protein in 52 cases of renal cell carcinoma and 14 cases of adjacent normal renal tissues was detected by immunohistochemical ABC method combined with microwave antigen retrieval and flow cytometry . Results: The positive rate and positive rate of c-yc protein in renal cell carcinoma were 76.92% and 65.45% ± 29.19%, respectively, which were higher than those of normal renal tissues by 14.29% and 8.59% ± 3.66% (P <0.005). c m yc in renal cell carcinoma 55.77% distributed in the cytoplasm, 21.15% distributed in the nucleus, while in normal kidney tissue are all distributed in the nucleus. The cytoplasm was higher in G2 and G3 than in G1 (χ2 = 9.244, P <0.005). The clinical stages T3 and T4 were higher than those in T1 and T2 (P <0.001). Conclusion: The cytoplasmic expression of c-y-yc promotes the occurrence and development of renal cell carcinoma, and is related to the degree of malignancy and clinical stage. It is an important indication of poor prognosis.