目的探索中国株庚型肝炎病毒（ＨＧＶ）基因的变异。方法采用ＨＧＶ中国株５’非编码区序列设计引物、逆转录套式聚合酶链反应法，检测中国不同地区血清中的ＨＧＶＲＮＡ，７份阳性产物采用ＡＢＩＰｒｉｓｍ３７７ＤＮＡ自动测序仪进行序列测定。结果测定的２ｌ３个碱基中，７株中国ＨＧＶ分离株与非洲株ＧＢＶＣ（ｕ３６３８０）序列同源性分别为８８．２６％、８５．９２％、８８．２６％、８５．４５％、８６．８５％、８５９２％和８８．２６％，与美国株ＨＧＶ（ｕ４４４０２）序列同源性分别为９２．０２％、８６．８５％、８６．６７％、８９．２０％、８９６７％、８９．６７％和９ｌ．５５％，而中国ＨＧＶ分离株间的同源性均高于９２．０２％。结论中国ＨＧＶ分离株基因型不同于美国报道的ＧＢＶＣ和ＨＧＶ亚型 Objective To explore the variation of HGV gene in China. Methods The primers of HGV 5 ’untranslated region (HGV) in China were designed and reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect HGV RNA in serum of different regions of China. Seven positive products were sequenced by ABIPrism 377 DNA sequencer. The results showed that the sequence homologies of 7 Chinese HGV isolates with that of the African strain GBV-C (u36380) were 88.26%, 85.92%, 88.26% and 85.45%, respectively, in 2l3 bases. 86.85%, 8592% and 88.26%, respectively. The homologies with the American strain HGV (u44402) were 92.02%, 86.85%, 86.67%, 89.20%, 89 67%, 89.67% and 9l. 55%, while the homology between Chinese HGV isolates was higher than 92.02%. Conclusion The genotypes of Chinese HGV isolates are different from the GBVC and HGV subtypes reported by the United States