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目的探讨纳米炭(CNP)吸附紫杉醇(PA)靶向化疗药物的作用机制及疗效。方法选取30例胃癌患者纳入研究,分为试验组和对照组,每组各15例。试验组术中先以纳米炭吸附紫杉醇行淋巴靶向化疗后再行手术,对照组直接手术。术毕即取胃癌周围淋巴结送病检,将病检证实有癌转移的淋巴结行TUNEL法检测细胞凋亡,用免疫组化法检测Bcl-2及p16的阳性表达。结果试验组淋巴细胞的凋亡指数[(28.6±5.2)]%明显高于对照组[(7.0±1.9)]%(P<0.05);试验组及对照组淋巴细胞第一站与第二站间Bcl-2和p16阳性表达均无统计学差异(均P>0.05);试验组淋巴细胞Bcl-2的阳性表达率(1 6.7%)明显低于对照组(5 6.7%)(P<0.0 5),而p1 6的阳性表达率(63.3%)明显高于对照组(23.3%)(P<0.05)。结论 PA-CNP混悬液能靶向作用于胃癌转移淋巴结,使Bcl-2表达下降及p1 6表达增加,从而促进细胞凋亡。
Objective To investigate the mechanism and effect of nano-carbon (CNP) adsorbing paclitaxel (PA) targeted chemotherapeutic drugs. Methods Thirty patients with gastric cancer were enrolled in the study and divided into experimental group and control group with 15 cases in each group. In the experimental group, paclitaxel was adsorbed by nanocarbon and then lymphatic targeted chemotherapy before surgery. The control group received direct surgery. At the end of surgery, lymph nodes around the gastric cancer were taken for pathological examination. Lymph nodes confirmed to have cancer metastasis by pathological examination were examined for apoptosis by TUNEL method. The expressions of Bcl-2 and p16 were detected by immunohistochemistry. Results The apoptosis index of lymphocytes in experimental group [(28.6 ± 5.2)]% was significantly higher than that in control group [(7.0 ± 1.9)]% (P <0.05) There was no significant difference in the expression of Bcl-2 and p16 between the two groups (all P> 0.05). The positive rate of Bcl-2 in the experimental group was significantly lower than that in the control group (6.7% 5), while the positive rate of p16 (63.3%) was significantly higher than that of the control group (23.3%) (P <0.05). CONCLUSION: PA-CNP suspension can target the metastasis of gastric cancer lymph nodes, decrease the expression of Bcl-2 and increase the expression of p16, thereby promoting cell apoptosis.