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动物细胞培养是生物技术的一个重要领域 ,因为培养的细胞可以用来分离纯化病毒、生产疫苗和药物。本实验在日本对虾肝胰腺细胞和血细胞原代培养的基础上 ,利用RNA DNA值进行不同pH值培养基的比较 ,认为对于血细胞pH 7 2为最适值 ,而对于肝胰腺细胞pH 7 6比较合适。同时利用光镜及电镜做了一些形态和结构上的观察。
Animal cell culture is an important area of biotechnology because cultured cells can be used to isolate and purify viruses, produce vaccines and drugs. In this experiment, based on the primary culture of hepatopancreas cells and blood cells in Japanese shrimp, RNA DNA values were used to compare the different pH media, which was considered as the most suitable value for the pH value of blood cells 72 and the suitable pH value for hepatopancreas cells . At the same time using light and electron microscope to do some morphological and structural observations.